https://rd.uffs.edu.br/handle/prefix/4583 2025-12-02T09:44:19Z https://rd.uffs.edu.br/handle/prefix/8947 Title: Estudo clínico-molecular de Chlamydophila psittaci em calopsitas de criadouro comercial: uma abordagem com PCR em pool para zoonose Author: Massa, Isadora Morais First advisor: Champion, Tatiana Abstract: Birds of the species Nymphicus hollandicus (Calopsita) are domestic animals commonly kept as pets and are therefore raised close to humans. Therefore, it is important to study the health status of these animals, especially when it comes to zoonotic diseases. The objective of this study is to detect contamination by Chlamydophila psittaci in birds from a commercial breeding facility using the Polymerase Chain Reaction (PCR) laboratory method, as well as to correlate the symptomatic or asymptomatic status of the disease, pointing out the main clinical signs presented by the affected birds. For this, 23 birdsfrom a commercial breeder were selected and blood samples, choana swabs, cloaca swabs were collected, in addition to visual assessment and detection of clinical signs compatible with the disease. With the study, we sought to identify the number of animals carrying Chlamydophila psittaci in the samples and whether they present any noticeable signs in a visual assessment. Of the 23 birds evaluated, 5 (21.7%) had ocular signs, 9 (39.1%) had changes in feathering, 2 (8.69%) had changes in feces and 3 (13.04%) had changes in body positioning. Of the 23 birds evaluated, 9 (39.1%) animals obtained a positive result in the detection of the bacteria, with 3 (13.04%) birds dying during the study, these with necropsy changes characteristic of chlamydiosis, but it was not possible to identify the presence of the bacteria, therefore 14 (60.1%) birds tested negative or suggestively. Birds that were contaminated with Chlamydophila psittaci received appropriate treatment after the study. It was observed through analyzes using a generalized linear model that in the present study there was no correlation between the different clinical signs and the detection of the bacteria in the bird. Publisher: Universidade Federal da Fronteira Sul Type: Dissertação 2025-08-06T00:00:00Z https://rd.uffs.edu.br/handle/prefix/8854 Title: Indicadores de genotoxicidade e pesquisa de hemogregarina em cágados (Phrynops geoffroanus) de diferentes ambientes hidrográficos do Parque Nacional do Iguaçu Author: Marques, Ana Letícia Rodrigues First advisor: Braz, Paulo Henrique Abstract: This study aimed to identify and quantify erythrocytes with genotoxic indicators and to assess hemogregarine infection in association with leech infestation in individuals of Phrynops geoffroanus. Freshwater turtles were sampled from four distinct hydrographic environments in the Lower Iguaçu River, Paraná, Brazil, including: two tributaries, the Silva Jardim River (SJr) and Floriano River (FL-r); and two river islands, Ilha do Sol (SOL-i) and Ilha do Geninho (GEN-i). A total of 56 animals were evaluated for indicators of genotoxic damage based on erythrocyte alterations, and 58 were screened for hemoparasites through blood smear analysis. Linear biometric data were recorded for all individuals, including maximum carapace and plastron length and width along with body mass, sex, and the presence of anatomical lesions or abnormalities. Blood was collected via dorsal cervical venous sinus puncture, and blood smears were stained using the May-Grünwald Giemsa method. To determine the frequency of micronuclei (MNs) and nuclear abnormalities (NAs), as well as the occurrence and parasitemia of hemogregarine, 3,000 erythrocytes were analyzed per animal. Mean MN frequencies varied slightly among the four sites (0.29 ± 0.63 in SJ-r, 0.38 ± 0.50 in FL-r, 0.25 ± 0.39 in SOL-i, and absent in GEN-i), with no significant differences (p = 0.32). NAs were observed in 32 of 56 individuals (mean frequency of 7.2 per 1,000 cells), also without significant variation among sites (p=0.81). Hemogregarina infection was highly prevalent (82.76%), though the mean parasitemia was only 0.33% of erythrocytes. No significant differences in parasitism rates were detected among sampling sites (p=0.18), but a positive correlation was identified between infection and body size (p<0.001). Leech infestation was recorded in 20.69% of turtles and was also positively correlated with both body size (p<0.001) and hemogregarine infection (p=0.043). The absence of spatial differences in genotoxic indicators or parasitological parameters may reflect the ecological buffering effect of Iguaçu National Park, which likely reduces contaminant influx from neighboring agricultural zones. At the same time, the presence of leeches suggests that conditions favorable for vector persistence are maintained within the park. These findings highlight P. geoffroanus as a valuable sentinel species for freshwater ecosystem monitoring and emphasize the importance of surveillance at the interface between protected and anthropogenically impacted areas. Publisher: Universidade Federal da Fronteira Sul Type: Dissertação 2025-08-11T00:00:00Z https://rd.uffs.edu.br/handle/prefix/8801 Title: Aspectos ultrassonográficos abdominais em camundongos hígidos Author: Cordeiro, Heloisa Vieira First advisor: Braz, Paulo Henrique Abstract: Mice (Mus musculus) are rodents widely used as experimental models in research due to their short lifespan, small size, ease of handling and reproduction, and anatomical and genetic similarities to humans. Despite their frequent use in science and also as pets, there is a lack of data in the literature regarding abdominal ultrasonography in this species. This study aimed to describe the qualitative and quantitative abdominal ultrasonographic features of healthy mice. Twenty animals (10 males and 10 females), aged between 5 and 12 months and weighing 25 to 36 g, were selected from the certified animal facility of UFFS, Realeza campus. Chemical restraint was performed using midazolam (5 mg/kg, IM) in the quadriceps femoris muscle, combined with physical restraint via dorsal skinfold. Trichotomy was performed from the xiphoid to the pubic region, followed by complete abdominal ultrasonographic evaluation using a commercially available portable ultrasound system with a multifrequency linear transducer (8–17 MHz). Parameters such as organ positioning, morphology, margin definition, echogenicity, echotexture, vascularization, anatomical relationships, wall layering, number of peristaltic contractions in 15 seconds, and echogenicity of biliary, urinary, and gastrointestinal contents were recorded. Quantitative measurements (in millimeters) included adrenal gland length and thickness (cranial and caudal poles), kidney length and width, ovarian and testicular dimensions, uterine horn and body thickness, seminal gland thickness, and wall thickness of the stomach, small and large intestines, spleen, urinary bladder, and pancreas. Images were acquired in longitudinal and transverse planes; for kidneys, a dorsal plane was also included. After ultrasonographic evaluation, animals were euthanized with an isoflurane overdose in a customized inhalation chamber. Four specimens (two males and two females) had their thoracic and abdominal cavities filled with 10% formaldehyde and were submerged in formalin for 48 hours. Organ measurements were then performed using a digital caliper, following the same ultrasonographic parameters. Additional specimens were fixed in 10% formalin for anatomical sectioning and photographic documentation. Comparisons between sexes and between ultrasonographic and anatomical data were conducted using statistical tests in GraphPad Prism® 8.0.2. Ultrasonographic measurements were consistent with anatomical findings, with no statistically significant differences between methods or sexes. The results provide a reference atlas of mouse abdominal ultrasonography, which may support veterinary sonographers, clinicians, and researchers in experimental and diagnostic applications. Publisher: Universidade Federal da Fronteira Sul Type: Dissertação 2025-07-21T00:00:00Z https://rd.uffs.edu.br/handle/prefix/8691 Title: Enriquecimento ambiental com feno de Tifton (Cynodon ssp.) ou "corrente" em baias de gestação coletiva para porcas: efeito sobre parâmetros produtivos, estresse e estresse oxidativo Author: Rocha, Jullian Rissardi da First advisor: Berenchtein, Bernardo Abstract: Brazil ranks fourth in global swine production, with 2,099,036 active sows in 2023 and a pork production of 5.156 million tons. Swine farming has a significant impact on the national economy, requiring harmonization between productivity and animal welfare (AW). This study evaluated the effects of environmental enrichment (EE) with hay on pregnant sows, comparing it to metal chains and a control group without EE, in accordance with Normative Instruction 113/2020. The experiment was conducted on a commercial farm in western Santa Catarina, using 96 sows of the Danbred 90 line, divided into three groups: control (without EE), T1 (chains), and T2 (hay). Zootechnical parameters (birth weight, weaning, daily weight gain) and biochemical parameters (cortisol, TBARS, antioxidants) were analyzed. The results indicated that T2 had a higher total birth weight (22.5 ± 4.12 kg; p < 0.05) and a significant reduction in cortisol and oxidative stress levels (p < 0.05), suggesting an improvement in BEA and productive performance. It is concluded that EE with hay is a viable strategy to promote welfare and reproductive efficiency, aligning with the regulatory and sustainable demands of swine farming. Publisher: Universidade Federal da Fronteira Sul Type: Dissertação 2025-02-21T00:00:00Z