https://rd.uffs.edu.br/handle/prefix/5423 2026-05-29T03:41:27Z https://rd.uffs.edu.br/handle/prefix/9334 Título: Inibição do gene CD73 em linhagem de câncer de bexiga: uma estratégia terapêutica de resposta a quimioterápicos que atuam na biossíntese de purinas Autor(es): Rosa, Vandriel Pedone da Primeiro Orientador: Maciel, Sarah Franco Vieira de Oliveira Abstract/Resumen: The ectonucleotidase CD73 has been widely recognized as an important modulator of the tumor microenvironment, mainly due to its role in the conversion of AMP into adenosine, promoting immunosuppression and favoring tumor progression. The present study aimed to correlate CD73 expression as a biomarker of cell viability in response to treatment with cell death– inducing chemotherapeutic agents, such as cisplatin, paclitaxel, and doxorubicin, in the T24 bladder cancer cell line, using a strategy of protein translation modulation through RNA interference, as well as a CD73 knockout T24 cell line. Gene expression analysis did not demonstrate increased CD73 expression in the T24 tumor cell line compared to a set of non- tumoral bladder tissue samples used as control. In contrast, protein expression showed a reduction after RNA interference treatment, confirming modulation at the transcriptional level. In cell viability assays, the isolated or combined application of chemotherapeutic agents in the unmodified T24 cell line did not show a significant effect. After CD73 silencing by RNA interference, a change in the drug response profile was observed. Combinations such as cisplatin + paclitaxel and cisplatin + doxorubicin resulted in a significant reduction in cell viability at all tested concentrations, indicating a potentiated cytotoxic effect under conditions of purinergic pathway modulation. In the CD73 knockout T24 cell line, the isolated application of paclitaxel and cisplatin did not promote a significant reduction in cell viability, while doxorubicin at the lowest concentration maintained a cytotoxic effect. In this cell line, combined treatments generally did not show consistent significant reductions in cell viability, suggesting that the absence of CD73 may alter the response to combination therapies. Overall, the results indicate that CD73 silencing or inhibition plays a relevant role in modulating cellular response in the presence of the chemotherapeutic agents cisplatin, doxorubicin, and paclitaxel, whether used alone or in combination, influencing resistance or sensitivity mechanisms depending on the experimental context and therapeutic strategy adopted. Thus, CD73 stands out as a potential biomarker and promising therapeutic target in bladder cancer Instituição: Universidade Federal da Fronteira Sul Tipo: Dissertação 2026-01-01T00:00:00Z https://rd.uffs.edu.br/handle/prefix/9326 Título: Qualidade de vida relacionada à saúde (QVRS) materna: relação com comprimento relativo dos telômeros da mãe e do neonato Autor(es): Dariff, Marina Suelen Trevisol Primeiro Orientador: Polettini, Jossimara Abstract/Resumen: Pregnancy induces physiological and psychosocial changes that can significantly affect both maternal health and newborn (NB) development. Telomeres — DNA-protein structures located at the ends of chromosomes — play a crucial role in genetic stability and protection against cellular aging. Evidence suggests that adverse conditions during pregnancy, such as stress, inflammation, and harmful lifestyle habits, may influence maternal health-related quality of life (HRQoL) and be associated with telomere shortening. The primary objective of this study was to describe sociodemographic, behavioral, health, and clinical characteristics, as well as to analyze the relative telomere length of maternal and newborn cells and its association with the HRQoL of postpartum women. A cross-sectional study was conducted at the maternity unit of Hospital Regional do Oeste (Chapecó, Santa Catarina, Brazil) between June 2024 and November 2025. Data collection included sociodemographic questionnaires, obstetric history, the EQ-5D-5L instrument with the Visual Analogue Scale (EQ-VAS) for self-rated health assessment, and oral mucosa swab sampling for telomere analysis by quantitative polymerase chain reaction (qPCR), expressed as the T/S ratio. Statistical analyses were performed using PSPP software, adopting a 5% significance level. A total of 131 mother-newborn dyads were included. Among the mothers, 78.6% were aged between 20 and 34 years, and 38.2% had low educational attainment. Smoking during pregnancy was reported by 11.5% of participants, alcohol consumption by 7.8%, and 19.1% reported maintaining physical activity during pregnancy. Regarding HRQoL, 74% of women reported high HRQoL perception prior to pregnancy, whereas 26% reported the same scores in the last trimester of pregnancy; 0.8% reported scores worse than death. In the EQ-VAS assessment, 30.5% of women rated their health as excellent before pregnancy, compared with 19.8% during the last trimester of pregnancy. With respect to telomere length, the median maternal T/S ratio was 0.65, while the median newborn T/S ratio was 2.11. No statistically significant associations were observed between maternal and newborn telomere length and HRQoL in the evaluated periods — pre-pregnancy and the last trimester of pregnancy (p > 0.05). These findings indicate a decline in perceived health during the last trimester of pregnancy and suggest that maternal factors may influence newborn telomere biology, although no statistically significant associations were identified in this sample. The results highlight the need for longitudinal follow-up and larger sample sizes to further investigate epigenetic mechanisms related to cellular aging processes during pregnancy. Instituição: Universidade Federal da Fronteira Sul Tipo: Dissertação 2025-01-01T00:00:00Z https://rd.uffs.edu.br/handle/prefix/9242 Título: Efeitos do estresse de privação maternal e isolamento social em comportamentos depressivos e ansiosos: potencial terapêutico dos óleos de Cannabis sativa em modelos animais Autor(es): Mingoti, Maiqueli Eduarda Dama Primeiro Orientador: Ignácio, Zuleide Maria Abstract/Resumen: Major Depressive Disorder (MDD) is a multifactorial disorder in which childhood traumatic events and chronic stress play central roles in the dysregulation of neuroplasticity. The resistance to conventional antidepressant treatments, which exhibit slow onset of action and limited efficacy in a significant portion of patients, has driven the search for new therapies, including the use of compounds derived from Cannabis sativa. This study investigated the effects of chronic treatment with full-spectrum and isolated cannabidiol (CBD) Cannabis sativa oils on depressive- and anxiety-like behaviors, as well as the hippocampal protein expression of cannabinoid type 1 receptors (CB1R) and brain-derived neurotrophic factor (BDNF) in a dual-stress model: maternal deprivation (MD) and social isolation (SI). A total of 110 Wistar rats (55 males and 55 females) were used. Animals were subjected to MD during the first days of life (PND 1–10) and SI during adolescence (approximately PND 50–80). The animals were divided into five groups: Control (C+CT); Stress + Vehicle (E+CT); Stress + Escitalopram (E+ESC; 10 mg/kg); Stress + Full Spectrum Oil (E+FS; 1 mg/kg); and Stress + Isolated CBD (E+CBD; 30 mg/kg). Treatments were administered orally for 14 days. Depressive- and anxiety-like behaviors were assessed through forced swimming and elevated plus maze tests, respectively. The results indicate that MD and SI induce depressive and anxious behaviors in rats. Isolated CBD (30 mg/kg) reversed these effects in males, while in females, both isolated CBD (30 mg/kg) and full-spectrum oil (1 mg/kg) were effective. Biochemical analyses of the hippocampus showed that, in males, the MD and SI protocol significantly reduced BDNF and CB1R levels. While none of the treatments were effective in restoring BDNF concentrations, treatment with isolated CBD and full-spectrum oil restored CB1R levels to control values. In contrast, in females, stress did not alter baseline BDNF or CB1R levels. However, interventions with isolated CBD (30 mg/kg) and full-spectrum oil (1 mg/kg) promoted a downregulation of BDNF levels in the female hippocampus, while full-spectrum oil (1 mg/kg) induced an upregulation of the CB1 receptor. Instituição: Universidade Federal da Fronteira Sul Tipo: Dissertação 2026-01-01T00:00:00Z https://rd.uffs.edu.br/handle/prefix/9155 Título: Perfil epidemiológico da sepse e choque séptico em um hospital do oeste do Estado de Santa Catarina Autor(es): Lima Júnior, Raimundo Nonato de Primeiro Orientador: Oliveira, Gabriela Gonçalves de Abstract/Resumen: Sepsis and septic shock constitute significant public health problems, ranking among the leading causes of hospital mortality worldwide, especially in intensive care units (ICUs). National data show a high mortality rate, exceeding 55% in public hospitals, a scenario that reflects structural inequalities, diagnostic limitations, and low adherence to standardized care protocols. Within this context, the study described the prevalence of sepsis and septic shock cases and characterized the clinical-epidemiological profile in patients hospitalized in a hospital in Western Santa Catarina. This is a quantitative study, with a retrospective and prospective design, of an observational and cross-sectional nature, with documentary analysis of medical records and data collection from nursing professionals, developed in a large public hospital located in the western region of the state of Santa Catarina, where 1,619 patients hospitalized between March 2020 and June 2025 were evaluated. Of the total, 26.7% presented sepsis, 28.8% progressed to septic shock, and 44.4% did not have a diagnosis of sepsis. During the study, a high proportion of septic shock was observed, indicating the severity of the cases treated and a possible delay in clinical recognition. The majority of patients were male (59.8%) and between 51 and 80 years old. Professions such as farmers, homemakers, and drivers were the main occupational activities, and most identified as Catholic. The racial distribution showed a higher prevalence of white people, a demographic characteristic of the region. In order to contextualize the theoretical review, the evolution of sepsis definitions was highlighted, from the SIRS criteria to Sepsis-3, emphasizing the importance of early identification through SOFA and qSOFA scores. The study analyzed 1619 ICU patients from March 2020 to June 2025, distributed as follows: 433 (26.7%) with sepsis, 467 (28.8%) with septic shock (CS), and 719 (44.4%) without sepsis, with no significant temporal variation (ANOVA). Demographically, men predominated (59.8%), the age range was 51-80 years, farmers (28.4%), housewives (13.4%), drivers (6.2%), and Catholics (75%), with predominantly white skin. Total organ dysfunctions amounted to 2854, led by metabolic (27.7%), pulmonary (26.7%), and renal (21.5%) disorders; Comorbidities in 2136 cases include hypertension (23.5%), cancer (12.9%), and diabetes (12.6%). Clinical signs (2348) highlight tachypnea (30.1%), tachycardia (29.7%), and hypoxemia (18.7%); laboratory tests reveal elevated CRP (98.7%), high urea (91.8%), altered lactate (84%), creatinine (67.9%), and thrombocytopenia (44.4%). Infectious foci (1124) are pulmonary (54.6%), with Klebsiella pneumoniae prevalent in tracheal aspirate (27.5%), Candida spp. in urine culture (37.9%), and Staphylococcus spp. in blood culture (43.6%); total antibiotics (2598) prioritize carbapenems (19.4%). Prognosis indicates a mortality rate of 52.6% in sepsis/CS (43.8% and 50.8%), with hospitalization of 2-20 days and a positive correlation (r=0.9973), in addition to the scarce application of prognostic scores (94.9% absent), aligning with the Brazilian literature on heterogeneity and the need for bundles. Instituição: Universidade Federal da Fronteira Sul Tipo: Dissertação 2025-01-01T00:00:00Z