Pré-tratamento e hidrólise enzimática da biomassa lignocelulósica de milho em reator batelada

Abstract

The recent need to diversify the energy matrix intensified research related to the use of renewable energies, such as those from agro-industrial waste. Therefore, this work had as objective to evaluate, in a batch reactor, the step of pre-treatment of the lignocellulosic biomass, aiming to the production of fermentable sugars. For this purpose, the Full Factorial Planning, 2², with triplicate at the central point, was used to evaluate the process variables "glycerin concentration" and "type of chemical agent". Then, enzymatic hydrolysis of the biomass was carried out, using the enzymatic complexes Ctec2 and Htec2, in percentages of 2 and 0.5% (v/m), respectively. It was worked with temperature of 50ºC and speed of agitation 8 of the equipment, for 24 hours. Through the planning, it was observed that glycerin had a negative effect on the process, while the addition of calcium hydroxide and calcium oxide resulted in a good reduction in the structural rigidity of the cell wall of the plant, obtaining a must with concentration of sugars around 0.31 gsugar/gbiomass, corresponding to approximately 47.4% yield relative to the amount of cellulose and hemicellulose fractions present in the biomass. In view of the positive response obtained, the residual corn biomass was shown as a promising raw material for the production of bioethanol. In addition, with regard to the use of mechanical agitation, using impeller of the naval type, it was possible to affirm that it maintained the homogeneous medium with the reason solid/liquid of 60 g/L, being favorable for the production of lignocellulosic bioethanol in a batch reactor.