Introdução in vitro de segmentos nodais de Guadua angustifolia Kunt

Abstract

The species Guadua angustifolia Kunt has raw material with high economic potential, but is still underutilized. The objective of this study was to establish a protocol for in vitro introduction Guadua angustifolia Kunt, aseptic utilized the next treatments: 30 seconds in 70% ethanol + 10 minutes in 2% NaOCl with 2 drops of Tween® 80; 30 seconds in alcohol 70% + 10 minutes in 0.1 HgCl2; 30 seconds in alcohol 70% + 30 minutes of immersion in 8 ml / l of PPM®; 30 seconds in alcohol 70% + 15 minutes in 10% H2O2 and 24 hours in Cercobim® 2g / l + 30 seconds in 70% alcohol + 10 minutes in 2% NaOCl with 2 drops of Tween® 80 in MS medium was kept in chamber of growth with photoperiod of 16 hours and temperature of 25 °C. After 14 days the variables explants contamination, asepsis and sprouting were evaluated. Contamination occurred in all treatments, being the treatment with HgCl2 the lowest contamination of explants and higher rate of aseptic explants. Still the treatments with HgCl2 and Cercobin® were those that stood out in the sprouts variable. However, oxidation of explants occurred in both cases.