Quantificação de micronúcleos e relação com o índice de massa corporal e variáveis preditivas para obesidade em mulheres

Abstract

Obesity is evaluated as a multifactorial disease and is currently considered the epidemic of the 21st century, which has reached significant levels in relation to public health. Cytogenetic abnormalities have been observed in obese individuals, with the micronuclei (MN) being one of these alterations. MN is defined as a small nuclear body, delimited by membrane and separated from the main nucleus. Using the MN test has advantages, including low cost and rapid analysis for screening large numbers of substances. The objective of this study was to evaluate the frequency of MN cell damage in exfoliated cells of the oral mucosa of women of two studied groups: obese and eutrophic. The sample came from the Support Group for Weight Loss and Welfare, assisted by the Municipal Health Department of the city of Capanema, Paraná, Brazil and made up of thirty-two people in total. The sample was evaluated according to the MN frequency per group, with BMI and personal health data obtained through a questionnaire. Cell collection was performed on both sides of the buccal cavity and later, by means of a smear technique, blades of the exfoliative material were made, which were read by light microscopy, in order to quantify the number of MNs found. After a statistical study it was concluded that the cells of the obese participants did not present a greater amount of MN than in the cells of the eutrophic participants. There is a need to complement and continue this and other studies that take into account and evaluate thevarious factors that are able to change the frequency of MN.