Genotoxicidade em células da mucosa bucal de usuários de cigarros e cigarros eletrônicos: teste de micronúcleo

Abstract

Objective: To investigate the effects of electronic cigarettes and traditional cigarettes on buccal mucosa cells among university users of these products, using the micronucleus test at the Federal University of Fronteira Sul, Campus Realeza/PR. Methods: This was an observational study employing both qualitative and quantitative approaches involving 24 individuals of both genders, over 18 years old, divided into three groups: smokers, non-smokers, and former smokers. Data regarding gender, age, and reasons for tobacco use were collected through a questionnaire. Genotoxic activity was assessed by analyzing micronucleus frequency. Buccal mucosa cells were collected using the conventional cytological method, stained with Gentian Violet for micronucleus analysis and detection of altered cells. For analysis, 500 cells per individual were counted, evaluating micronucleus frequency and cellular alterations. The data were statistically analyzed. Results: The analyzed data indicate a difference between non-smokers and tobacco smokers (both traditional and electronic cigarettes) concerning micronucleus formation and cellular alterations.Conclusion: This study revealed a higher frequency of cells with micronuclei and cellular alterations in the group of tobacco and electronic cigarette smokers, indicating genotoxicity