Introdução in vitro de lavanda (Lavandula angustifolia)

Abstract

Lavender (Lavandula angustifolia) belongs to the Lamiaceae family which it has great economic value due to extraction of essential oils that can it used to food and cosmetic industries and also in aromatherapy regulated by international legislation. This work aimed to establish in vitro plants introduction from lavender seeds. The experiment was carried out by seeds asepsis in a 70% alcohol solution for 3 minutes, followed by an treatment in a sodium hypochlorite solution at concentrations of 0%, 1%, 2%, 4% and 6% during 20 minutes followed by a triple rinsing in sterilized distilled water. The seeds were inoculated on MS medium (Murashige and Skoog, 1962) in petri dishes and cultured into a BOD germination chamber for a period of 28 days. After that the variables analyzed were germination and contamination rates. The averages obtained between the treatments were compared using the Tukey 5% test. According to the results obtained for the variable germination, the best results were founded when used 1%, 2% and 4% of sodium hypochlorite solution with averages of 16, 16 and 20% but not statistically different where detected from each other. For the contamination variable, the treatments 2% and 6% of NaClO represented the best results and no bacterial/fungical contaminations were observed. It is concluded the efficiency of treatments containing sodium hypochlorite for the in vitro plants introduction of Lavandula angustifolia.