Ocorrência de nematoides entomopatogênicos em áreas de cultivos na agricultura familiar

Abstract

The isolation of entomopathogenic nematodes (EPNs) in agricultural family farming areas is crucial for understanding the effectiveness of these organisms in pest control. This analysis allows for the comparison of the presence and activity of nematodes in different agricultural areas, providing insights for integrated pest management and the sustainability of crops. The aim was to analyze soil samples from various agricultural activities in family farming were positive for the presence of nematode pathogens (EPNs). Soil samples were collected from three different areas: Area 01 – fruit cultivation (jabuticaba); Area 02 – fruit cultivation (establishment); and Area 03 – horticulture. After collection, laboratory analyses were conducted on the soil samples, where they were subjected to larvae of Tenebrio molitor to observe potential infections. Finally, isolations were performed, which could be confirmed in repetitions regarding the occurrence of the nematodes. Soil moisture was measured for comparison with the presence or absence of EPNs. For molecular analysis, the captured isolates were sent to the State University of Northern Paraná. Following molecular analysis, the main species identified was Oscheius tipulae, which represented over 93%. The moisture levels in the three areas were 25.60%, 20.60%, and 21.20%, respectively. It was concluded that positive samples with EPNs could be collected from different cultivation areas in family farming.