Estudos pré-tratamentos e hidrólise enzimática do bagaço de cevada para produções de açúcares fermentescíveis

Abstract

With the increase in industrial production large quantities of waste are generated which in addition, bring extra costs for businesses can contaminate the environment if they are not a correct destination. Among them is the barley residue which is produced in large quantities and can be used to second generation ethanol. The feasibility of using this waste only becomes interesting if we get a dry product with large amounts of sugars available for fermentation. Thus, this study aimed to evaluate the bagasse drying kinetics and verify the effectiveness of physical pretreatment on enzymatic hydrolysis by obtaining the reducing sugars present in biomass. Within the studies it observed that the barley bagasse has moist 75.24% being necessary to perform the drying step in order to preserve it for a longer period. To evaluate the effect of varying agitation, reduction in size and steam explosion, we used the technique factorial design experiments and response surface analysis. Among the variables investigated, the excitement and the particle size showed the significant effects in obtaining fermentable sugars. In contrast, steam explosion showed a negative effect. This effect can be explained by the low temperature (121 ° C) employed in the process. The maximum concentration of reducing sugars was 10.98 g L-1, obtained in the following experimental conditions: crushed barley bagasse (material that passed through 18 mesh sieve); with agitation (100 rpm) and; without steam explosion. Thus, it becomes apparent that the combination of pretreatment allows a higher yield in the extraction process of total reducing sugars (TRS) and barley residue has the potential to be used in the production of biofuels.