Avaliação da atividade de peroxidases e lipases em diferentes sistemas reacionais

Abstract

This study investigated different strategies in order to increase the activity of oxidative enzymes and lipase (commercial and noncommercial). To this end, the enzymes were subjected to treatment in an ultrasonic bath and a microwave in order to evaluate the effect of these systems as well as power and temperature using experimental design. Peroxidases enzymes extracted from rice bran and soybean meal when treated in ultrasound showed an activity increase (129.2% 147.89% FA and FS), and for the commercial enzyme, the increase in activity was not unimpressive. It was also observed changes in the behavior of lipase enzyme as its hydrolytic activity, since there was obtained an increase of approximately 300% enzyme activity relative to enzyme activity compared to without treatment with ultrasound. The data obtained in this study suggest that the treatment with microwaves is a reaction medium for conducting reactions catalyzed by peroxidases because it was possible to obtain gain in activity tests. The current literature shows a lack of studies that evaluate the changes in terms of activity and enzyme stability after exposure to ultrasound and microwave. Only a few scientific papers were find showing that the proposed work is extremely relevant to the area of environmental sciences, since the enzymes under consideration can be apply at different reactions, such as degradation of mycotoxins and effluent treatment.