Processos fermentativos em resíduos agroindustriais utilizando a levedura yarrowia lipolytica qu69

Abstract

The constant development of biotechnology and the search for processes in which it is possible to add commercial value to previously discarded products, the possibility arises of the use of agroindustrial residues in bioconversion processes. These processes are performed by microorganisms and have the advantages of low cost, easy control and non-use of toxic chemicals. The Yeast such, Yarrowia lipolytica was used in bioconversion processes for the production of various products of industrial interest. To estimate the number of cells present in the inoculum that will be used in fermentative processes, the two most used methods are the McFarland scale and the Neubauer chamber, which were originally developed for counting bacteria and blood cells, respectively, subjective. In order to improve the yeast cell counting methods Yarrowia lipolytica QU69, was use in bioprocesses the McFarland scale associated with UV-Vis spectrophotometry and used to estimate the number of cells present in suspensions. The method proved to be very fast, reliable and reproducible, and could be applied in routine analyzes and classroom experiments. In the bioconversion process using yeast cells Yarrowia lipolytica QU69 the biomass produced was evaluated by the production of proteins and lipids with several carbon sources from agroindustrial waste and the process was optimized. Significant values were obtained when using manioc as a carbon source, urea-containing supplementation medium and a process conducted under dubnoff shaking at 100 rpm and a temperature of 40 ° C.