Atividade antifúngica do óleo essencial de Melaleuca alternifolia sobre fitopatógenos do mamoeiro

Abstract

The objective of this work was to evaluate an antifungal activity of the essential oil (OE) of Melaleuca alternifolia on phytopathogenic fungi of papaya culture, through mycelial growth tests for anthracnose (Colletotrichum gloeosporioides) and target spot (Corynespora cassiicola), followed by germination of spores for smallpox (Asperisporium caricae), in direct and indirect methods of application of OE in culture medium. In the direct mycelial growth test, OE was incorporated into the BDA culture medium, and in the indirect test it was placed on the lid of the Petri dish with gauze. They were evaluated as rules of 0; 0.05; 0.1; 0.5 and 1% of the OE. No center of each plate was peaked in a 5 mm mycelium discotheque, placed in B.O.D. and reached the average diameter of the colonies, until reaching a greater reach ¾ of the plate. For the spore germination test and germ tube length, a suspension was prepared and added to a microscope slide containing the culture medium. The experiments were conducted in a completely randomized design with 4 replications. The results show the potential of tea tree OE in the control of phytopathogens, both in mycelial growth and spore germination, the antifungal effect increased as there was a greater concentration in direct and indirect application, inhibiting completely from 0.5%.