Avaliação da atividade antioxidante e antitumoral de diferentes extratos de Physalis peruviana l

Abstract

Physalis peruviana L. (PP) belongs to the Solanaceae family, and has aroused interest due to the long list of chemical constituents of nutritional and pharmacological interest. Extracts obtained from different parts of the plant have exhibited antioxidant activity, immunomodulatory activity, antitumor activity, anti-inflammatory activity, antidiabetic activity, and antimicrobial activity. The bioactive compounds present in plant extracts may vary due to several factors such as, their complexity, the solvent and extraction methods, applied quantification methods, in addition to the stages of plant maturation. In this sense, the objective of this work was to evaluate the phenolic compounds, antioxidant and antitumor activity of different PP fruit extracts at different stages of maturation. We observed a significant difference in phenol content and antioxidant activity between fruit maturation stages in all analyzed extracts. A significant difference was observed in the extracts obtained with different solvents. All extracts showed significant antitumor activity, however, the most significant result was observed in the highest concentrations of the hexane extract, where we obtained a greater cytotoxicity in SK-Mel-28 cells, and a significantly greater increase in the viability of PBMCs cells. The analysis of pro-oxidative and antioxidant parameters showed an induction of oxidative stress, triggering a lipoperoxidation in the lipids of the membrane of SK-MEL-28 cells and an increase in the generation of nitrite oxide (NO), thus inducing apoptosis of cancer cells.