Tratamento alternativo de sementes de beterraba com indutores de resistência

Abstract

To ensure seeds and seedlings with high physiological and sanitary quality resulting in a uniform plant stand free of harmful pathogens, procedures that can improve the seed's potential and its tolerance and resilience, such as the use of resistance inducers, need to be studied. Several treatments have been shown to be effective in inducing resistance and enhancing physiological quality, such as bacterial strains that colonize the rhizosphere, applications of ethylene, chitin, chitosan, and silicon. Therefore, the objective of this work was to verify the treatment effect of beetroot (Beta vulgaris L.) seeds of the Vermelha Comprida cultivar with different resistance inducers on physiological performance and sanitary quality. The experiment was carried out in a completely randomized design to analyze the effect of resistance inducers on the physiological potential and health quality of sugar beet seeds, and a randomized block design to evaluate the effect of resistance inducers on the health of sugar beet seedlings. The treatment of beetroot seeds was carried out with the following treatments: control (distilled water only), Salicylic acid, Bacillus amyloliquefaciens, Trichoderma harzianum, and Chitosan, with five replications. After coating, the seeds were subjected to the following evaluations: germination percentage, seedling length, seedling dry mass, Blotter Test, quantification of proteins in plant tissues, and determination of phenylalanine ammonia-lyase activity. To interpret and compare the different doses used for each inducer in isolation, the data obtained were submitted to analysis of variance and regression test. From the data analysis, there was a significant positive effect on the physiological potential of seeds treated with salicylic acid and Bacillus amyloloquefaciens; there was a significant effect of all the evaluated inducers for the variables of seedlings with pre-emergent Damping-off and incidence of Fusarium sp. and increase in Phenylalanine ammonia-lyase activity in seeds treated with T. harzianum. Furthermore, there was a reduction in total protein content in seeds treated with salicylic acid, B. amyloloquefaciens and T. harzianum.