Detecção de colonização por Helicobacter pylori em cavidade oral por técnica de reação em cadeia da polimerase

Abstract

to evaluate the prevalence of Helicobacter pylori in the oral cavity, as well as its possible association with sociodemographic and clinical aspects. Methods: this is a cross-sectional study. The study included adult patients of both sexes who answered a standardized questionnaire for the study and had an oral cavity sample collected by scraping of oral mucosa with cytological brush. The samples were tested for positivity for H. pylori using the nested Polymerase Chain Reaction (PCR) technique, which was monitored by electrophoresis in 2% agarose gel with ethidium bromide and visualized under ultraviolet illumination. For the analyses, the level of significance adopted was 5%. Results: a total of 93 patients were tested for H. pylori, the prevalence found was 49.5%. A significant association was found between positivity for H. pylori and smoking (p=0.024) and a protective association with the recent use of proton pump inhibitor drugs (p=0.034). Conclusions: Helicobacter pylori is present in the oral cavity of a significant portion of people, this may represent a form of infection and failure in the eradication of the bacteria in the stomach.