Qualidade toxicológica e microbiológica da água de poços artesianos do município de Realeza Paraná

Abstract

The municipality of Realeza is located in southwestern Paraná, a large part of the population makes use of water from artesian wells. Thus, this work aimed to analyze the cytogenotoxicity of water from rural and urban artesian wells in the municipality of Realeza - PR, using the Allium cepa system. To perform the analyzes, water was collected from artesian wells, which were randomly chosen, following the list of records present in the City Hall / Sanitary Surveillance, and samples were collected from three wells in the urban area and three in the rural area of the municipality. Allium cepa bulbs were exposed to water samples collected in triplicate, with positive control in distilled water, and negative control in 0.0006% aqueous copper sulfate solution for 72 hours, then the roots were removed with the hood. , with a size of approximately 2 cm in length. Subsequently, the collected root meristems were exposed to the Carnoy fixative for 24 hours, washed and rehydrated, the slides were prepared and exposed to the Aceto-hydrochloric Orcein solution, heated and put to rest. After staining, the meristematic regions of the roots were separated on the lamina, covered with one or two drops of acetic acid. Changes in Mitotic Index and Chromosomal Changes were observed. The obtained data were submitted to statistical analysis by chi-square test test with reliability of 5%.The results suggest that water from artesian wells has the potential to cause damage to the genome of eukaryotic organisms.