Caracterização de biomateriais a partir da padronização de modelos experimentais de scaffolds dérmicos caninos

Abstract

Regenerative Medicine consists of a multidisciplinary area that aims to promote the regeneration of tissues that have suffered injuries. In the case of the skin, when lesions occur that cause extensive tissue loss, one of the main forms of treatment is the use of an autologous skin graft, but it is not always possible to collect the skin for this treatment. In view of this, Tissue Engineering, a subarea of Regenerative Medicine, began to develop several methods for the production of biomaterials, such as scaffolds, that can replace the skin in these grafts. Therefore, this study aims to evaluate the effectiveness of a dog skin decellularization protocol for the production of canine dermal scaffolds. The protocol used was based on the immersion of canine skin fragments in different solutions to promote cellular tissue removal. These samples were separated into three groups and subjected to specific washing processes for each group, which consisted of immersion in PBS, NaCl or EDTA. Subsequently, a decellularization protocol common to all groups was performed. This decellularization was based on immersion of the samples in 1% SDS and 2% Triton X-100. At the end of the process, the samples were submitted to histological analysis in HematoxylinEosin for evaluation and characterization. Through this histological evaluation, it was possible to notice the absence of cell nuclei in the tissue. In addition, no changes were observed in the conformation of collagen fibers in the extracellular matrix. Therefore, it can be concluded that the protocol used in the present study promoted significant cell removal, indicating efficiency in decellularization without causing structural changes in the tissue.