Avaliação do efeito do tratamento com Glifosato e Round-up® em linhagens de Glioma

Abstract

Glioblastomas (GBMs) are Grade IV primary gliomas that can affect all age groups, being more frequent in the adult population and those close to 60 years of age. With a poor prognosis, this neoplasm is characterized by highly invasive cells of the nervous system, with excessive proliferation and resistant to the few existing treatments. Life expectancy for patients with GBM can reach months, even after surgery, radiotherapy and chemotherapy. Thus, this study assumed that the use of pesticides is linked to the incidence of tumors, with the hypothesis that Round-up® and Glyphosate (GLY) have cytotoxic effects on GBM strains, having on their cellular effect. GLY is a broadspectrum, post-emergence herbicide, potentiated with mixtures of surfactants, which facilitate the absorption of the compound by plants. Round-up® is the best known GLYbased herbicide on the market. In this work, we used the GBM strains A-172 (human) and C6 (rat) to perform different tests varying the exposure time to treatments (24, 48 and 72 hours; 28 days) and GLY and Round- procedures. up® (0.0025%, 0.005% and 0.01%), with the objective of evaluating a compound cytotoxicity, verifying the potential of acute and chronic treatments, and the induction of senescence, a process that stands out as an antitumor process. We demonstrated for the first time that Roundup® and GLY have cytotoxic effects on GBM strains, decreasing cell viability, cell number, colony formation and population doubling, mainly at the 0.01% dose of Round- up ®. We did not find results obtained in relation to the induction of senescence by the treatments used. The results also showed that these effects were more pronounced when performing treatment with Round-up® than with GLY.