Análise da resposta imunológica de anuros como biomarcador de alterações frente a exposição a agrotóxicos

Abstract

Atrazine is among the most polluting organisms and the main target ecosystems among the organisms that affect the main ones have already been found in water. In ecological research, a count of leukocyte numbers aids in the study of vertebrate responses when exposed to stress. In this way, an investigation of how amphibians react to natural or induced changes, such as the introduction of pesticides in their habitats, is important to verify possible population declines. Therefore, the use of glyphosate and atrazine pesticides was tested, which can interfere with the number of leukocytes, in order to verify whether this analysis can be used as a differential biomarker for immune responses. For this, an evaluation of the effects of devices produced by chronic exposure was carried out as a change in the leukocyte profile under laboratory conditions and then a comparison was made between the results of the two herbicides. For the Brazilian natural trials, between the doses of natural tadpoles and glyphosate allowed by Brazilian legislation, between 2 and 2 and 65 and 1000 μg a.e/L of glyphosate, per. seven days. Subsequently, 10 μL of cardiac puncture blood were taken from each use, the euthanized treatment was removed, where blood samples were taken, after 10 μL, after blood collection, and stained to perform the 10 otic leukocyte count, after blood collection, after blood collection from 10 optics, under lens, under lens. 100x objective. The tadpoles a commercial formulation of exposure and glyphs showed differences in leukocytes. In the blood of gyrons treated with atrazine there was an increase in lymphocytes, eosinophils, but an increase in basophils. Those exposed to glyphosate had a higher number of lymphocytes, monocytes and neutrophils and a lower number of basophils. Neutrophil/lymphocyte ratios tend to increase only in the two with the highest glyphosate. An exposure to reduction of cells and especially a reduction of cells to a prominent profile, suggesting configurations of leukocytes as a form of increase or number of lymphocytes and the other leukocytes indicating an increase in the susceptibility of the animal to environmental changes with the defense of the organism, indicating that this method can be used as a biomarker.