Avaliação dos constituintes do sistema purinérgico e parâmetros inflamatórios em linfócitos de pacientes com adenocarcinoma colorretal esporádico

Abstract

Among the pathological changes that increase the invasive potential of tumors, the evasion of the inflammatory response and purinergic signaling emerge as important components of the progression of colorectal cancer. The cellular immune response is closely associated with the presence of inflammatory infiltrate in the tissue, composed of mononuclear cells that proliferate and produce pro and anti-inflammatory cytokines. In this infiltrate, cell communication mediated by nucleotide/nucleoside receptors is modulated by ectonucleotidases, which hydrolyze ATP, ADP and AMP to adenosine. ATP can act as a pro-inflammatory molecule, while adenosine acts as a negative feedback to limit inflammation. IL-17, synthesized by the group of Th17 lymphocytes, is a pro-inflammatory cytokine that can be found in serum. The objective of this work was to evaluate the influence of purinergic signaling in the regulation of the lymphocyte-mediated inflammatory process, by determining the activity of ectonucleotidases involved in the metabolism of ATP, ADP and adenosine in lymphocytes, and quantifying the levels of IL-17 in the serum of 27 patients with colorectal cancer and 21 healthy participants, respecting the application of a informed consent form. The clinicopathological information of patients who participated in the research prior to the beginning of any type of treatment for the disease were also collected and considered. Using colorimetric methods, an increase in the activities of NTPDase and ADA enzymes in lymphocytes was observed, also, a reduction in the amount of serum IL-17 in patients, representing an adaptive consequence of the patient facing the advanced stage of the disease. Thus, it is suggested that the alterations found in patients promote an anti-inflammatory state, which favors tumor progression.