Avaliação da viabilidade celular e do efeito antioxidante dos ácidos cafeico, clorogênico e rosmarínico em PBMCS de sujeitos com mutação no Gene CFTR

Abstract

Cystic fibrosis (CF) is an autosomal recessive genetic disease caused by a mutation in the cystic fibrosis transmembrane conductance regulator gene (CFTR), which encodes a protein of the same name, responsible for the transport of chloride and bicarbonate. The reduction or absence of protein function leads to some multisystem disorders, in addition to chronic lung infections, caused mainly by Pseudomonas aeruginosa, and an exaggerated immune response, in which neutrophils appear as the majority cell type, although they are not able to eliminate pathogens. properly. The presentation of the disease depends on numerous factors and the same genotype can be clinically different in different subjects. As alternatives to treatment, CFTR modulators have shown important results, however, there are limitations arising from the genetic background that may reduce their effectiveness. From this perspective, phenolic compounds emerge as a natural and cheaper therapeutic strategy to control inflammation and oxidative stress. To evaluate the action of caffeic, chlorogenic and rosmarinic acids, we collected peripheral blood from three subjects with CF and from three unaffected individuals. Peripheral blood mononuclear cells (PBMCs) were grown and expanded in cell culture flasks and transferred to 24-well plates. After transfer and adaptation, they were treated with different concentrations of the studied acids. Cell viability was analyzed by the MTT method and oxidative stress parameters were also tested. We identified that phenolic compounds increased ascorbic acid concentrations and nitric oxide levels, as well as increased cell viability. In this sense, phenolic compounds are more accessible and natural strategies for the treatment of CF that should be studied.