Identificação de SNPs (SINGLE NUCLEOTIDE POLYMORPHISMS) para Podridão Radicular de Fitóftora (Phytophthora sojae) em uma Combinação Biparental de Soja (Glicine max)

Abstract

The phytophthora root rot of soybean, caused by Phytophthora sojae is widespread in the main producing countries world's soy, and can cause plant death in extensive areas. Through breeding methods has been the development of cultivars with resistance genes to this disease, requiring the use of tools for the identification of genetic variation between individuals in a population. This study aimed to identify SNPs for evaluation of phytophthora root rot (PRF) in soybean line from the cross between cv. NS 6909 IPRO and INT 001, is resistant to PRF and susceptible respectively, using techniques such as molecular analysis and testing in greenhouses with plant exposure to the pathogen. To obtain the data were analyzed 291 plants segregating F2 generation through DNA collection for identification of SNPs by real-time (PCR). These results were compared with the test field, carried out in a greenhouse, the company Nidera Seeds Realeza PR, which were made inoculations of the pathogen through the technique of "toothpick" colonized with the fungus, for this we used a bulk Phytophthora sojae races more infect soybean crops in southern Brazil, used by the company Nidera seeds in tests in the breeding program. The results indicated that the marker GM03_456359 [G / A] is effective in resistance to phytophthora root rot in the lines from the cultivar NS 6909 to phytophthora root rot.