Biocompatibilidade in vivo de scaffolds placentários bovinos em camundongos

Abstract

Decellularized matrix scaffolds can be used in Regenerative Medicine as a therapeutic strategy for tissue and organ regeneration or replacement. The use of these biomaterials has achieved positive results, given the biological advantages inherent to their origin. One of the main challenges is the limitation of sources for its production, which to a certain extent makes its use on a large scale unfeasible. In this sense, researchers have sought alternatives with greater availability, such as fetal attachments. Among these, scaffolds derived from bovine cotyledons stand out, which after decellularization maintain structural integrity and high concentrations of extracellular matrix. Despite presenting themselves as promising alternatives for tissue engineering purposes, more detailed studies of their in vivo application and interaction with the host are still needed. Therefore, the present project pre-clinically tested, in mice (Mus musculus), Swiss strain, the tissue inflammatory reaction and collagen deposition at the interface between subcutaneous tissue and biomaterial. Analyzes were performed through macroscopic assessment of the implantation site on predetermined euthanasia and assessment days. After a general evaluation of the macroscopic structure and the inflammatory reaction, it was possible to observe with this study that the bovine placenta scaffold is a promising alternative for tissue engineering, since it did not cause tissue rejection, and can be used as a biomaterial for tissue reconstruction.