Análise de componentes do sistema purinérgico em mulheres com câncer de mama

Abstract

Breast cancer (BC) arises from a deregulation in the cell cycle that favors the abnormal growth of cells in various breast tissues, and constitutes a global public health problem due to its high incidence and high mortality rate. It is known that the purinergic system acts by modulating the immune responses in several diseases and this is the subject of several studies regarding the CM. In this bias, the present study sought to evaluate the amount of systemic Adenosine Triphosphate (ATP) and the enzymatic activity of CD39 through the hydrolysis of ATP and Adenosine Diphosphate (ADP). The study was composed of women with BC (n= 36) and controls (n= 33) matched for age and sex. Statistical analyzes were performed using the GraphPad Prism 8 software, the data were tested for normality (Kolmogorov Smirnov), after which the data were tested using the Student's T test or the Mann Whitney test. For correlation analysis, Pearson's test was used. It was demonstrated that the amount of ATP is greater in patients compared to the control group (p=0.0001) and that CD39 activity does not show variation between groups. There was a negative correlation between ATP quantity and hydrolysis in patients (p=0.0008) which was not found in controls (p=0.7052). Furthermore, a positive correlation was observed between ATP and ADP hydrolysis in both groups (patients: p=0.0262; and controls: p<0.0001). Therefore, it was evident that there is a high concentration of systemic ATP in patients compared to controls and that this molecule, in addition to promoting inflammation, also acts in purinergic signaling. These data suggest that the purinergic system is modulated in order to maintain high ATP concentrations in order to modulate the inflammatory response by stimulating it, however, it is necessary to perform analyzes of the other components of the purinergic system to elucidate the pathways and use this modulation as a therapeutic target.