Análise de DNA do aparelho bucal de tabanídeos para detecção de Trypanosoma evansi

Abstract

Trypanosomoses are globally significant diseases for humans and animals. Horses affected by trypanosomiasis, transmitted by Trypanosoma evansi, present a condition known as "surra" or "mal das cadeiras," which can cause paralysis of the hind limbs. It is important to detect the parasite using molecular techniques such as PCR to detect T. evansi DNA in the mouthparts of tabanid flies, the parasite's vectors. In the Laranjeiras do Sul region, Paraná, as well as throughout Brazil, there are very few studies on T. evansi and tabanids. For this study, tabanid flies were collected from selected properties in the Laranjeiras do Sul region, Paraná, and taken to the biochemistry laboratory at the Universidade Federal da Fronteira Sul (UFFS), where DNA was extracted from the mouthparts of the two most commonly collected tabanid species, and PCR was performed to detect T. evansi.