Assepsia de sementes de Ocimum Basilicum l. (manjericão-roxo) e introdução in vitro do cultivar

Abstract

Ocimum basilicum L. (Lamiaceae) the basil, is an aromatic plant with great importance, used in several segments due to its essential oil, linalool and anthocyanin content. In biotechnology, the culture of cells and plant tissue used in micropropagation is important for the genetic improvement of this species. The research aimed to establish an in vitro micropropagation protocol for purple basil, the seeds were immersed in 70% alcohol pre-treatment for 3 minutes, then sodium hypochlorite concentrations of 1%, 2%, 4% and 6% were used for 20 minutes, and distilled water for control treatment, to determine the influence on germination rate and contamination. In vitro introduction was performed with 6% sodium hypochlorite treatment. For statistical analysis, Tukey's test was used at 5% probability level. The contamination values were not significant, since itsoccurrence was restricted to the control treatment (66.6%). For the germinated seeds variable the mean comparison test showed that the highest rate occurred in the control treatment (89.67%), but due to contamination this treatment is not indicated. The best treatment for the disinfection of the seeds was with a concentration of 6% of sodium hypochlorite, where 28.17% of the seeds germinated after 28 days, the other concentrations showed no statistical difference between them, presenting the highest average of 5.67%. In the in vitro introduction the germination rate was 65.71% without contamination, with an average height of 4.3 cm (aerial portion) and 2 cm (root).