Efeito do exercício físico nas ectonucleotidases em linfócitos de pacientes hemodialíticos

Abstract

Introduction: Purinergic signaling in the kidney occurs through complex mechanisms. It is known that renal injury leads to an increase in ATP concentration, contributing to the inflammatory responses in Chronic Kidney Disease (CKD). In addition, ADA, the enzyme that converts adenosine into inosine, also contributes to an increased inflammation profile. Resistance physical exercise, marked by muscle contraction, increases the production of antioxidants, with an anti-inflammatory effect, therefore, it can be used as a non-pharmacological therapeutic resource for several chronic diseases. Objectives: evaluate the effect of resistance physical exercise on inflammatory markers of the purinergic signaling in patients with CKD. Methods: This is an interventional study with a quantitative approach and a descriptive and comparative nature, using a before-and-after design in 30 patients undergoing hemodialysis at the Clínica Renal do Oeste (CRO). The evaluation was carried out through the determination of ectonucleotidases’ (CD39, CD73 and ADA) activity involved in the metabolism of ATP, ADP, AMP and adenosine, and quantification of extracellular ATP, in the lymphocytes of these patients before and after the practice of the exercise. Results: An increase in the amount of extracellular ATP, a reduction in the hydrolysis of ATP and AMP, and an increase in the activity of the ADA enzyme were found after the resistance exercise protocol. The results obtained evidence that the practice of physical exercise led to a pro-inflammatory condition in these patients. Conclusion: The practice of physical exercise in patients with CKD influenced the inflammation biochemical markers of the purinergic system in lymphocytes. Therefore, it is safe to say that more studies are needed to provide a broader and clearer view of the short- and long-term effect, as well as low and moderate intensity, of physical exercise on the purinergic system.