Atividades das ectonucleotidases em linfócitos e parâmetros inflamatórios em pacientes hemodialíticos após a realização de um protocolo de exercícios e auriculoterapia

Abstract

Auriculotherapy (AUR) and RPE are low-cost and easy to apply therapies, they can provide numerous benefits to patients with CKD, both in management and prognosis and improvement in the quality of life of individuals, and studies using these therapies are scarce, which shows the importance of further studies on these treatments. We evaluated the effect of AUR and resistance physical exercise (RPE) on the activity of ectonucleotidases in lymphocytes of 96 patients with chronic kidney disease (CKD) on hemodialysis, divided into the groups: AUR, RPE and AUR+RPE. The activity of NTPdase1, ecto5'nucleotidase, adenosine deaminase (ADA) in lymphocytes and the quantification of ATP and cytokines in the patients' serum were analyzed before and after the interventions. There was an increase in glomerular filtration (GF) in patients in the AUR (p=0.0171) and RPE (p=0.0272) groups. The AUR protocol promoted an increase in ATP hydrolysis (p=0.048) in lymphocytes and a reduction in the quantification of ATP in the patients' serum. The RPE group showed a reduction in the hydrolysis of ATP (p=0.0016), ADP (p = 0.001) and AMP (p = 0.004) in lymphocytes, a reduction in interleukin 6 (IL6) levels (p=0.040) and greater activity of ADA (p=0.017). We concluded that AUR promoted changes in the individuals' purinergic system, with possible anti-inflammatory effects, while RPE promoted changes in the purinergic system that characterize pro-inflammatory, antithrombotic, anticoagulant and cardioprotective effects.