Capacidade de formação de biofilme em isolados de mastite

Abstract

Mastitis is the main disease that affects dairy herds, with an expensive cost to dairy farming, and its connection with public health, since pathogens are transmitted that can cause diseases in humans. Its multiple etiology is a problem for control, as it can be derived from physiological, traumatic, allergic, metabolic and/or infectious origins. The pathogens involved in mastitis can form biofilms, interfering with food quality and damaging dairy production. The biofilm can be understood as a sessile community, linked by a substrate and incorporated by a matrix of polymeric and extracellular substances. Thinking about the importance of biofilm formation, the study aimed to evaluate 42 samples of microorganisms to verify the ability to form biofilm in isolates in cases of mastitis. The methodology was used to form biofilms in 96-well microplates, inoculating in suspension of 5 mL of BHI, at 37º for 24 hours, these samples were taken to tubes with 10 mL of 0.9% saline solution, and standardized in a spectrophotometer. 100 microliters of TSB, 1% glucose and 2 microliters of already standardized sample were added to each well. To visualize the formation, the plates were washed three times with distilled solution, fixed with methanol, stained with crystal violet, and washed with distilled water, which were re-solubilized in acetic acid to resuspend, and read in a spectrophotometer (λ = 605 nm). Biofilms were classified as weakly forming, strongly forming and negative. The negative control was performed with Staphylococcus epidermidis ATCC 12228 and the positive control Staphylococcus aureus ATCC 6538. The presence of biofilms on milking equipment, resulting from inadequate cleaning, represents a present source of contamination for milking animals, and for the industry, interfering both with animal health, as well as the quality of the milk produced. Eliminating biofilm is arduous, and has been increasingly addressed in the literature due to its current importance.