Avaliação do efeito antimicrobiano e antineoplásico do extrato de macela (Achyrocline satureioides) puro e diluído em ferro dextran

Abstract

Achyrocline satureioides (Lam.) DC is a medicinal plant native to South America, frequently used in ethnomedicine in several south american regions, despite being rich in bioactive compounds, studies on its antimicrobial and antitumor potential are still incipient. In view of the above, the present study aimed to determine the action of the aqueous extract of A. satureioides - obtained by infusion, on the strain of Pseudomonas aeruginosa (ATCC® 27853TM) and on the human melanoma cell line (SK-Mel-28 ). The extract was characterized according to its phytochemical composition (dosage of total phenolic compounds, flavonoids and high-performance chromatographic analysis) and its in vitro antioxidant activity (using three different assays: DPPH•, ABTS•+ and FRAPP method). The antimicrobial effect was determined using an antimicrobial sensitivity test (minimum inhibitory concentration, disk diffusion and Punch Hole technique) and the antitumor action was evaluated using assays for cell proliferation, cell migration, determination of cell death and apoptosis, production of reactive species of oxygen and nitric oxide, impact on levels of protein and non-protein thiols, as well as on the expression of ectonucleotidases CD39 and CD73. It was concluded that the A. satureioides extract showed antipseudomonal and anti-melanoma activity, which was potentiated in the presence of iron dextran. The unprecedented nature of such a finding is emphasized.