Sêmen bovino congelado e descongelado acrescido de extrato natural (NP), com potencial antioxidante

Abstract

Sperm cryopreservation allows rapid genetic advancement in commercial herds, especially in cattle, due to its use in reproductive biotechnologies such as artificial insemination, fixed-time insemination and in vitro embryo production. However, the process of freezing and thawing semen can cause cellular damage, especially due to the excessive generation of reactive oxygen species (ROS), which compromise sperm viability. This study evaluated the in vitro and in vivo viability of cryopreserved semen added with a natural extract (NP) with antioxidant potential, after freezing and thawing. The dissertation is organized in the format of a scientific article, which is described in chapter 01. Based on the results, it was possible to demonstrate that the percentage of motile and progressive spermatozoa was higher in fresh semen, with values ​​of 87.57% and 43.77% respectively, and the percentage of static spermatozoa was lower in the fresh control group, with 12.42%. The kinetic parameters of speed, movement and trajectory did not differ between treatments after thawing. No differences were observed in the values ​​of individual and total defects between the groups evaluated, as well as in the percentage of intact, semi-damaged and damaged spermatozoa. Regarding the pregnancy rate, the partial descriptive results indicated 70.59%, 77.78%, 50.00% and 70.59% after TAI using semen belonging to groups 1, 2, 3 and 4, respectively. It is concluded that the addition of the natural extract with antioxidant capacity in the semen diluent subjected to cryopreservation did not harm the movement kinetics, sperm viability and/or pregnancy rate.