Produção de enzimas a partir de biocompósito de trichoderma koningiopsis em biorreator

Abstract

The growing demand for sustainable processes has increased interest in the production of microbial enzymes with potential industrial and environmental applications. This study evaluated the production of amylase and cellulase enzymes by the fungus Trichoderma koningiopsis in an Airlift bioreactor. The fungal extract was obtained via submerged fermentation in a defined culture medium, with samples collected at 24 and 48 hours. The results demonstrated high enzymatic production, especially during the first 24 hours, highlighting the biotechnological potential of the fungus. The highest activity was observed for amylase, whereas cellulase remained stable throughout the fermentation process. The Airlift bioreactor proved efficient for enzyme production due to its adequate oxygen transfer, lower mechanical stress on fungal mycelium, and its potential for industrial-scale up. Therefore, the obtained results demonstrate the potential of Trichoderma koningiopsis for applications in sustainable bioprocesses, plant biomass biodegradation, and the development of bioproducts associated with the circular economy.