Otimização da extração de compostos fenólicos por meio de técnica sustentável

Abstract

Jabuticabeira is a native plant from South America, which produces edible fruits. Native plants have been promising for the development of extracts due to their bioactive compounds beneficial to health, especially in fruits. However, research on plants leaf properties are scarce. So, the extraction of bioactive compounds are selective, as it requires propert extraction method and solvent affinity with sample matrix. Within the range of green solvents, the natural euthetic solvents (Nades), characterized by non- toxic, biodegradable and provide high yields, stand out. In addition, sustainable methods such as ultrasound assisted extraction are regarded as emerging methodologies, which promote rapid extraction of less environmental impact. Therefore, the objective was to study the phenolic compound kinetics of jabuticabeira leaves through ultrasound assisted extraction and then optimize extraction parameters by performing a factorial multivarious planning 23. The extraction kinetics was performed with 0.75g of sample (M), 60% of sonator amplitude (A) and 20% of Nades, and the optimization was studied with 3 central points and 2 axial points ranging M (0.25-1.75 g), A (30-90 %) and Nades (10-30%) for the concentration of total phenolic compounds (CFT). Thus, after 8 min an efficient extraction was obtained and in later times a linearity remained. Thus, fixing the time of 8 min it was determined that the mass parameters of the sample, amplitude, and concentration of the solvent results in a variation between 32.22 ± 0.19 to 233.31 ± 4.35 mg AGE/g of dry jabuticaba leaves. However, through the surface curves and the contour curves, a tendency was observed to optimize extraction. Therefore, the lowest proportion of amplitude, amount of mass and Nades concentration resulted in greater extraction, approximately 233.31 mg AGE/g of jabuticaba leaves.